Minimum number of myosin motors accounting for shortening velocity under zero load in skeletal muscle
نویسندگان
چکیده
منابع مشابه
Rabbit papillary muscle myosin isozymes and the velocity of muscle shortening.
Rabbits, ages 4-24 weeks, were injected with saline or thyroxine (150 micrograms/kg) for 7 days, and force-velocity curves were generated using papillary muscles from these hearts by a method described previously. In addition, the structure and relative amounts of myosin isozymes from papillary muscles and from 3- to 5-mg segments of the left and right ventricular free wall were analyzed by pol...
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Skeletal muscle can bear a high load at constant length, or shorten rapidly when the load is low. This force-velocity relationship is the primary determinant of muscle performance in vivo. Here we exploited the quasi-crystalline order of myosin II motors in muscle filaments to determine the molecular basis of this relationship by X-ray interference and mechanical measurements on intact single c...
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We have shown that myosin light chain phosphorylation inhibits fiber shortening velocity at high temperatures, 30 degrees C, in the presence of the phosphate analog vanadate. Vanadate inhibits tension by reversing the transition to force-generating states, thus mimicking a prepower stroke state. We have previously shown that at low temperatures vanadate also inhibits velocity, but at high tempe...
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Skeletal muscle's ability to shorten and lengthen against a load is a fundamental property, presumably reflecting the inherent load-dependence of the myosin molecular motor. Here we report the velocity of a single actin filament translocated by a mini-ensemble of skeletal myosin approximately 8 heads under constant loads up to 15 pN in a laser trap assay. Actin filament velocity decreased with ...
متن کاملIntraflagellar transport velocity is governed by the number of active KIF17 and KIF3AB motors and their motility properties under load.
Homodimeric KIF17 and heterotrimeric KIF3AB are processive, kinesin-2 family motors that act jointly to carry out anterograde intraflagellar transport (IFT), ferrying cargo along microtubules (MTs) toward the tips of cilia. How IFT trains attain speeds that exceed the unloaded rate of the slower, KIF3AB motor remains unknown. By characterizing the motility properties of kinesin-2 motors as a fu...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 2016
ISSN: 0022-3751
DOI: 10.1113/jp273299